Life Sciences CN

Fluorescence Lifetime Flow Cytometry (FLFC)

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Figure 1: Schematic of a fluorescence lifetime flow cytometry setup. A pulsed laser excites a fluorescent sample. Emitted photons (blue, light red, dark red) are filtered by spectrum and collected by single-photon detectors. A time-to-digital converter (Time Tagger) correlates the laser trigger with photon arrival times to measure fluorescence lifetime histograms. These fluorophore-unique histograms enable fluorophore discrimination, even in the case when spectral filters fail due to spectral overlap.

Dynamic Light Scattering (DLS) Particle Size Analysis

Next-generation multi-angle DLS turnkey design with built-in consistency checks, raw photon access, and intelligent spike filtering for fast, reliable particle sizing.

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Figure 1. The image consists of two main parts: the top half is a schematic of a Dynamic Light Scattering (DLS) setup, and the bottom half shows the DLS analysis and comparison between large and small particles based on the measured data. Top: A laser source emits a monochromatic light that travels horizontally and enters a transparent sample container (a cuvette). Inside this cuvette, several blue particles are shown in random “Brownian” motion. A Single Photon Detector “SPD” is situated at an angle θ from the laser propagation to collect the scattering data from the sample. The correlator acquires and calculates the correlation of the timing data from the detectors in real time. A cable connects the correlator to a computer “PC”, where data analysis is performed. Bottom: Visualization of the different behavior of small and large particles in solution, represented as blue circles (dark and light blue, respectively) and with their hydrodynamic radius (Rs and RL, respectively). Three different analysis plots are shown underneath, from left to right: Left: Intensity vs. Time (s): Two jagged lines represent the fluctuations in light intensity over time.The large particle has slower and smoother fluctuations in light blue color. The small particle shows faster fluctuations in dark blue color. Middle: Autocorrelation Function (ACF) vs. log 𝜏 (s): This plot shows two autocorrelation curves that represent the diffusion behavior of particles suspended in the examined solution. The large particle's curve decays more slowly. The small particle's curve decays faster, reflecting quicker diffusion. Right: Intensity vs. Size (nm): A graph with two sharp Gaussian curves. The left peak is labeled Rs for the small particle. The right peak is labeled RL for the large particle, indicating a size distribution derived from the correlation data.

Fluorescence Lifetime Imaging (FLIM)

Discover fluorescence lifetime imaging (FLIM), a powerful imaging technique for mapping fluorescence lifetimes with picosecond precision. Learn how Swabian Instruments' Time Taggers enable high-resolution FLIM measurements using advanced timing electronics, supporting detectors like PMTs, SPADs, and SNSPDs for cutting-edge research applications

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Fluorescence Lifetime Imaging (FLIM)

Time-Resolved Photoluminescence (TRPL)

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A schematic diagram of a Time-Resolved Photoluminescence (TRPL) setup. A Pulse Streamer pulses the laser, which then excites a sample. The emitted photons are collected by a single-photon detector (SPD), and the arrival times of both the laser trigger and the detected photons are time-stamped by the Time Tagger. The time stamps are streamed to a PC, where analysis is performed to create a plot of intensity over time (lifetime).
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